View/Open - 宮崎大学

宮崎大学学術情報リポジトリ
Title
Author(s)
クルマエビ血球が産生するフリーラジカルに関する基礎
的研究
伊丹, 利明; 吉田, 照豊
Citation
URL
http://hdl.handle.net/10458/4544
Date of Issue 2005-03
Right
Description
学術雑誌掲載論文の抜き刷りは、出版社に著作権許諾が
得られていなのため未掲載
平成16年度‾平成17年度科学研究費補助金(基盤研究
(c))研究成果報告書
'J )vxI.t:::'Jtn~fJ~~~9
~7IJ-7~:h)vl::
[email protected]~UJf~
16580155
!lIP! 16 ~~~!lIP!
17 ~~'4~UJf~*iDJJ1i
(~.UJf~ (C)) UJf~P!!l.i5-.
UJf ~ it ft ~ i1Pl- 'IJ B~
-g_7\:~ .~$ ~t§
<r1LfJ(~>
? )l/xI~(J)~11"'1' )I,., A ltnnE (PAV) ~r1L.d)cT~iTft~~€Er1. ~j1g~(J)I
c'.Ji~¥r::~:1\:tJWi~~~~.~<(J)ft~i*~i*¥~JJ!¥r::~(,
\i6.lvt3.o
rio B*(J)? )l"'~IC'~~r::f3(,\-cr1.
t=.C7t..
BB~ 63 ~r::t:-?ctJL)~ra'*~ 3,000 t--~(J)
~j£~(f.)[ft=.fJ(. PAY (J)§@~Lt=.3¥/iX 8 ~,::r1*~ 1,500 t--~c~j£.r1*~Lt=.0
i-(J)~. ~j£[email protected]]flLt=.tJ(J)(J). 2,000 t--~(::cc'*,?-C(,\~o
*t=.. B *r1ttt
~ ~tJ~~(J)IC'.A~~(f.)L). ttt~(J)4JiIC' ~j£(J)*~ 1/3 r::(f.)t=.~*~ 25
~.±r::.WJ7:;7fJ\a;.AL -c(, \~o
rnt ~ L-C.
•
LfJ\L.
n
t--~
~j£~~(J)~~~§@r::~~~jJt~~fJ(
A I C' fJ\ a; (J) tit ~ ~ It fJ(~ tf:j ~ n t=. ~ CfJ(:1\: ~ <_
~ ~ n~ tJ c'.
4Ji
I~~(J)~(J)~~11ii~fJ(~tA(J)ilactJ'?-c('
\~o
~ (J) J: ? tJ iT R~ ~ nE (J) J( ~ (J)
rnt ~ (J) 1 '::> r::. 4Ji lJ ~ (J) ~ 1t c i- n r:: i~? I c'
(J)~~ItJj~tm:1J(J)ittl'fJ(~~a;n~o ~na;~r&~T~t=.d)r::.Jill.t.l(J)r&~~(J)
n L) f.Ddj. ~tit~ ~ 1t~'1l~tJ(, \ ~~~.;!~JtJ C r::~ ~ ItJjflPtm:1J (J) rRl.t. ~1t ~ ~
~(J).MJJl:t;~r1f!~fJ(-~LtJ(, \tJc'~<(J)rQ,as ~m7t.. -c(, \~o ~(J)Jjji~(J)
,::>r::.
Ic'm(J)iltJlm.ctJ~~Mlt¥JtJIIJ~1J~!fJ(tJ(,
? )I,., ~ I C' (J) ~ i* 1tJj~1m (J)
m. c L-C.
\~cfJ((f.) [fa;n~o
ltn~ (J):It ~;! 11.
11 f3 J: u: ltn~ (J) :iE 1t 11 r:: '::> (, \ -C. 15- Lt=. 0LfJ\ L.
:7 I
/ -
) I,.,:t
1
JJG I:< r1,
=f- ~~. - if;!
~ n i; (J) ;! 11 r1 iifl t¥J tJ ~ If ;!
'0
11~~"'1')I,.,A;!11~~TtJ(J)~r1tJ<.ra'flt¥JtJ-m~(::~~tJ(, i-~~, *~m
r::f3l, \-cr1~L)iiflt¥JtJJiiJl~~~tJ'::>:7IJ-7:;:t.J)I,.,'::~~~ 3-c-c.
i-(J)~tf:j1J
~!. j£~~ilf. ~i*j1g~(J)It.J1mtJc~B~a;fJ\r::L-c. ~i*ltJjfil]tm:1J(J)~fafir::r,c;mL
J:?cT ~tJ(J)~(f.)~o *_15-=1='1. 3¥/iX
16 ~J.lf3J:U: 17 ~J.l(J)/iX~~*cd)t=.tJ
(J)~(f.)~o
-1-
if~f.1l.
if~+tfi%: @:Pt*,JB"ij (rg~:1\:~ "~$ ft~)
if~~\.~'§%:
EEl lftltl (rg"':1\:~ "~$ l1Jft~)
a
(~g.iU:F9)
~i~;~~g
~fil16 ~~
~fil17 ~~
t,l}=t
,"C.,a
iiJlftJl
ra,mftJl
A=t
~~
1,700,000
1,700,000
3,400,000
0
0
0
1,700,000
1,700,000
3,400,000
if~~fi
(1) ~~it~
1.
Itami, T., Mekata, T., Maeda, M. , Yoshida, T. Detection of superoxide and nitric
oxide in kuruma shrimp marsupenaeus japonicus hemocytes. UPV Journal of
Natural Sciences, 10(1), 135-144, 2005.
2.
Maeda, M., Kusumoto, K., Mizuki, E., Itami, T., Ohba, M. Long-term
maintenance of primary ovarian cultures from the kuruma shrimp,
Marsupenaeus japonicus. Fish Pathology, 40(4), 187-190, 2005.
3.
Maeda, M., Mizuki, E., Itami, T., Ohba, M. Replication of white spot syndrome
virus in ovarian primary cultures from the kuruma shrimp, Marsupenaeus
japonicus. Journal of. Virological Methods, 116(1), 89-94, 2004.
(2) DRI~fi
1. Itami, T., Mekata, T., Maeda, M., Yoshida, T. Novel detection methods of
superoxide and nitric oxide in kuruma shrimp Marsupenaeus japonicus
hemocytes. 7th Asian Fisheris Forum 04, Penang, Malaysia, Nov. 30-Dec. 4,
2004
2.
Itami, T., Mekata, T., Maeda, M., Yoshida. Detection of superoxide and nitric
oxide in kuruma shrimp Marsupenaeus japonicus hemocytes. 6th Symposium
on Diseases in Asian Aquaculture, Columbo, Sri Lanka, Oct. 25-28, 2005
3.
Itami,T., Yoshida, T., Mekata, T., Hirono, I., Aoki, T. The biodefense system
and its activation with immunostimulants for the control of disease in shrimp.
International Symposium on Nutrition &Feeding in Fish 2004, Phuket, Thailand,
May, 3-6, 2004.
-2-
(3) tfj~Ji~
1.
i¥ft*IJBJl 71<#
~. Ri*~~ rIt::'.JI]~*J. 7J<j£*±(J't~:). 276 ]l2004
~
-3-
-p-
o~~atP~~)..\1c..::JrJi'2i.~~ '»1ft o-=.f.~
a}e;t=?~~~~¥t ')"\1[ti~¥f l~W!ld ~ltU~u~8~p ~±~IF*iI=f(~*$~~Wo-=.f.~~"¥~=?CD'l\ 1:P+4i+Ja~~:r::HI¥¥f.~~
·8
'~\e;t
=? ~ -=.f. c.. \e;t:P+ +J a IiII +J ~~ ~ CD ij, =f( ~ 1( ~ ~ f.- (I c:. ~:r:: J'ff ~ CD rl¥¥f. ~ ~ .L
0-=.f.c..:P+=?\e;t~
((B}e;t=?~~~~r,*dlt}e;tij, =f(~CD1(~~f.- (Ic:. ')..c..:r::J~~'t:'1( rY~ 't:' /l--l't:'
o
}e;t=f(~'la~s~~
·9
-=.f.c..:P+=?\e;t ~ ((B}e;t=? ~ ~~~f!~
91 ~~HI~ '}e;t~~~=f(~+J• • 'l::J~m~;HI~ '+J*$~~W-
·S
o-=./.c..:p+
=?\e;t~((Ehe;t=?~~~a~!t}e;t~r:rrc:.-~~""'i1:rseSdl
''VV\ld '+J)"1=?rl¥~H~
.p
0-=./.c..:P+=?\e;t~ ((B}e;t=?~~\ 1)"1Ihe;t~d'V+J::J ~¥t1(~~f.~
~t],~f:~i1:rse'Vl~+J::J~¥t,~""'+t~~-~/-'t:'
'z-~'Va+J::J~¥t*$~~W-
of
0-=./.c..:P+=?\e;t~ ((B}e;t=?~\ 1:P+~a ~¥t+J::J!H3!+J
a¥fsse!..I~~.l8N-=.t\ 1).. ~~\ 1 [ti*~ '+J)"1=?~[ti~¥tCD1(~~f.-(u~::'
0-=.tc..:P+::J~:r~~~~}e;titi~-=.t1~~
=?~~~a:f:Y ~liIfIlWJCD.~::Jll-
'(1:P+=?-f$l}e;t~¥i~
'+J ~ r~CD~liIfIlWJCD~
:rCDliIfllWJ=m:~~~~liIfllWJ.i~~[(;CD ,;:II~1(
4-
·z
'~\e;t
0-=.t1 J;[!H~\e;tlilfIlWJ4l
':P+~0i::J ~¥tCD1(~~f.-(I&~::'
°
I
~mt.l9~~
0-=.t1=?fX1 EI ~~ltCD±~~CD*j;
19~*$~~W-
'),,1 EI ~::J*$~H;-
'::J~~ o~ [email protected];~~r,tCD1(~~f.-(Ic:.
~+Jse::J~~~'t:'1( ""'yi1:rSf!~:f&lJ't:'/l~'t:'
'te~¥~.~fm$=f(~:r::J'ff~*n¥
¥f.~~ '(1*~::J~¥tCD.~~¥tCD1(~~f.-(Ic:.=?J;[!HCD~.i~liIf~~¥t
::JfXI~~w
0-=.t1=?fX1 EI ~=?~~ ~J;[!H~¥f.£t:
y~j1:rSf! [ti3JIl~::JfXI~Jl
~¥tCD1(~~f.-(Ic:.c..'l~.~'t:'1(
'::JC1l-=.t~ ~::J\e;t~((B~±~~fm~~=f(CD;:II~1(
'+J
"...
4-
fXIE1CD~~