Recovery of the genetically selected broiler line by transferring cryopreserved circulating PGCs. Atsushi Tajima 1) 2), Guy F. Barbato3), Palmer G. Cramer1), Carol H. Crouch 3) , Takashi Kuwana 4), Roy H. Hammerstedt 1) 1) Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA, 16802, USA. 2) Institute of Agriculture and Forestry, , University of Tsukuba, Ibaraki 305-8572, Japan. 3) Department of Poultry Science. Penn State University, University Park, PA, 16802, USA. 4) National Institute for Minamata Disease, Kumamoto 867-0008, Japan. Materials and Methods 1 Fertilized Eggs Incubation (42L) (37.8 oC) Blood collection (Stage 13-15) Freezing LN2 Bicell (Nihon Freezer) 10% DMSO, -80 oC Materials and Methods 2 Brown Leghon (BL) Eggs Dekalp, IL Incubation (37.8 oC) Blood removal (stage 13-15) Injection Hatch Progeny testing Thaw blood (Ice water 4 oC) Isolation of PGCs Remove DMSO Results Female BL(42L) Batch Total number of chick hatched Number of white chick 1 4 4 100.0 2 2 1 50.0 3 5 3 60.0 White chicks % Results Male BL(42L) Batch Total number of chick hatched Number of white chick White chicks % 1 12 6 50.0 2 21 10 47.6 Conclusion A broiler line (42L) was recovered using frozen/thawed circulating PGCs. Genetically important lines (grand parents, transgenics etc) can be recovered using frozen/thawed PCGs Acknowlegments Dr. Lary Vint (DeKalp) for providing Brown Leghorn eggs Financial support Ministry of Education, Science, Culture and Sports, Japan. Ministry of Environment, Japan.
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