Special Seminar of Environmental Analysis, The pretreatment

Special Seminar of Environmental Analysis,
The pretreatment method of environmental samples, and
the principle of LC/MS/MS”
Representative: Makoto YASOJIMA
Date: April 22, 2009
Place: Graduate School at Shenzhen, Tsinghua University, China
Organized by the Global COE Program “Global Center for Education and Research on Human
Security Engineering for Asian Megacities”
Co-organized by Kyoto University EML Program “International Center for Human Resource
Development in Environmental Management”
Invited Persons: None
Number of Participants: 20
Participants: 吴红利(Wu Hongli),吴江平(Wu Jiangping),孟凡花(Meng Fanhua),彭翱宇(Peng Aoyu),刘
振华(Liu Zhenhua),何艳丽(He Yanli),陶益(Tao Yi),陈磊(Chen Lei),靳军涛(Jin Juntao),杨云安
(Yang Yunan),龚浩(Gong Hao),曹鹏飞(Cao Pengfei),徐科(Xu Ke),钟润生(Zhong Runsheng),张
玉云(Zhang Yuyun),郭建宁(Guo Jianning),李明远(Li Mingyuan),周素珍(Zhou Suzhen)(Graduate
School at Shenzhen, Tsinghua University),水野忠雄,管運涛(Kyoto University)
The Shenzhen base so far has built structures and
relations required to launch research. Now we are
planning to conduct research regarding comprehension
of the current state of water contaminants in water
environment, and sophistication of water-purifying
system employing membrane filtration. Meanwhile,
Chinese water environment is supposedly extremely
polluted, and to understand the behavior of
infinitesimal compounds, such as endocrine-disrupting
chemicals and PPCPs, we need to pay close attention
when performing analyses. However no professor is
expert in environmental analysis in Graduate School at
Shenzhen, Tsinghua University, thus students do not
have an opportunity to learn pretreatment methods
and measuring procedures. They have no knowledge
on LC/MS/MS, which they will probably use in their
future studies; hence it is necessary to provide them
with proper information. This seminar intended for all
the students at Graduate School at Shenzhen,
Tsinghua University, and also staff from Monitoring
Centre to master the following: pretreatment methods
of environmental samples and their application, and
measurement principles of LC/MS/MS.
Achievement and Results
At this seminar, the participants received
explanations of kinds and methods of pretreatment
required to analyze micro pollutant contained in water
samples, and also the measurement principle of
LC/MS/MS. Some of the methods were familiar to the
participants, but there were others that they had not
heard of before, making the participants realize a wide
range of choices for pretreatment. Moreover, reference
was made to a combination of a suitable pretreatment
method and a target compound’s physical properties
and chemical structure, providing basic knowledge
regarding a choice of a suitable method of
pretreatment. The second half of the seminar was
devoted to explain the measurement principle of
LC/MS/MS expecting the newly equipped tandem mass
spectrometer (MS/MS) would be used as LC/MS/MS.
Although some participants had had experience of
using GC/MS as mass spectrometry technology, it was
necessary to give the information because the
measurement principle of GC/MS differs from that of
LC/MS/MS. The participants showed some but limited
understanding of the structure of an interface, an
ionization part, and the principle of fragmentation,
probably because it was the first seminar for them.
Therefore in addition to this, there is a need for
another seminar that they acquire hands-on experience
of using LC/MS/MS.
Many participants attended the seminar with interest
GCOE 特別セミナー 第一回環境分析技術セミナー 「環境試料の前処理方法と LC/MS/MS の測定原理」 代 表 者 : 八十島誠 開 催 日 時 : 2009 年 4 月 22 日 開 催 場 所 : 清華大学深圳研究生院 L 棟 109 主 催 : 京都大学グローバル COE プログラム「アジア・メガシティの人間安全保障工学
拠点」 共 催 : 京都大学 EML プログラム「環境マネジメント人材育成国際拠点」 招 聘 者: なし 参 加 人 数 : 20 名 主 な 参 加 者 : 吴红利(Wu Hongli ),吴江平(Wu Jiangping ),孟凡花(Meng Fanhua),彭翱宇(Peng Aoyu),刘振华(Liu Zhenhua),何艳丽(He Yanli),陶益(Tao Yi),陈磊(Chen Lei),
靳军涛(Jin Juntao),杨云安(Yang Yunan),龚浩(Gong Hao),曹鹏飞(Cao Pengfei),
徐 科 ( Xu Ke ), 钟 润 生 ( Zhong Runsheng ), 张 玉 云 ( Zhang Yuyun ), 郭 建 宁 ( Guo Jianning),李明远(Li Mingyuan),周素珍(Zhou Suzhen)(清華大学深圳研究生院),水
野忠雄,管運涛(京都大学) 目 的 ・ 概 要 深圳拠点ではこれまでに,研究に必要な体制・関
ン類や PPCPs などの極微量物質の挙動を把握するた
用する可能性の高い LC/MS/MS については,全く知
方法とその適用,②LC/MS/MS の測定原理を習得す
ることを目的とする. セ ミ ナ ー の 様 子 ・ 得 ら れ た 成 果 本セミナーでは,水試料に含まれる微量汚染物質
種類や方法,さらには,LC/MS/MS の測定原理につ
た に 配 備 し た タ ン デ ム 型 質 量 分 析 計 ( MS/MS) を
LC/MS/MS として使用することを想定し,LC/MS/MS
質量分析技術として複数の参加者が GC/MS の使用経
験を持っていたが,GC/MS と LC/MS/MS では測定原
LC/MS/MS をオペレートしながら指導を行う必要性
が示唆された. 講義に真剣に耳を傾ける参加者